Biodegradation of harmful industrial dyes by an extra-cellular bacterial peroxidase

Nowadays the treatment of environmental pollutants such as synthetic dyes (used in multiple industries paper, textile, food, plastic and pharmaceutical) has received much attention, especially for biotechnological treatments using both native artificial enzymes. In this context, many enzymes have been reported to efficiently perform dye degradation. Peroxidase is one enzyme, which causes degradation either by precipitation chemical structure aromatic or opening up their ring structure. present study an extra-cellular peroxidase extracted from a bacterial strain Bacillus sp. F31 JX984444.1 was tested its capability decolorize 16 different used various industries. Out textile decolorized 5 out 4 triphenyl methane (Basic Fuchsin (BF), Rhodamine B (RB), Coomassie Brilliant Blue (CBBG) Malachite Green (MG) showed decolorization 95.5%, 70.8%, 70% 40%, respectively, while polymeric heterocyclic Methylene (MB) 66.2% decolorization. These were studied further enhance after purification optimizing reaction conditions (temperature, time, enzyme concentration, buffer pH, concentration effect salt ions, H2O2 concentration). This indicates that extracellular (purified) can be useful tool (degradation/decolorization) industrial effluents contaminated with harmful dyes.